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In-vitro Antifungal Activities of Kombucha Tea Culture Supernatant Combined with Voriconazole against Vulvovaginal Candidiasis Clinical Isolates

 
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1. Title Title of document In-vitro Antifungal Activities of Kombucha Tea Culture Supernatant Combined with Voriconazole against Vulvovaginal Candidiasis Clinical Isolates
 
2. Creator Author's name, affiliation, country Rasha H. Bassyouni; Medical Microbiology and Immunology Department, Faculty of Medicine, Fayoum University, Fayoum 63514,; Egypt
 
2. Creator Author's name, affiliation, country Fatma AboElnaga Ahmed; Medical Microbiology and Immunology Department, Faculty of Medicine, Fayoum University, Fayoum 63514,; Egypt
 
2. Creator Author's name, affiliation, country Ahmed A. Ismaiel; Botany and Microbiology Department, Faculty of Science, Zagazig University, Zagazig 44519,; Egypt
 
2. Creator Author's name, affiliation, country Abdelsamie Abdelmoneim; Obstetric and Gynecology Department, Faculty of Medicine, Fayoum University, Fayoum 63514,; Egypt
 
2. Creator Author's name, affiliation, country Haitham Badran; Obstetric and Gynecology Department, Faculty of Medicine, Fayoum University, Fayoum 63514,; Egypt
 
2. Creator Author's name, affiliation, country Mazen A. El Zahry; Obstetrics and Gynecology Department, Faculty of Medicine, Azhar University, Cairo 11651,; Egypt
 
2. Creator Author's name, affiliation, country Reham Ali Dwedar; Medical Microbiology and Immunology Department, Faculty of Medicine, Cairo University, Cairo 12613,; Egypt
 
2. Creator Author's name, affiliation, country Ahmed Ashraf Wegdan; Medical Microbiology and Immunology Department, Faculty of Medicine, Cairo University, Cairo 12613,; Egypt
 
3. Subject Discipline(s)
 
3. Subject Keyword(s) antimicrobial; biofilm; Kombucha tea culture; voriconazole; vulvovaginal candidiasis
 
4. Description Abstract

Objective: To investigate the antifungal activity of voriconazole, with and without Kombucha tea culture, against Candida strains isolated from vulvovaginal candidiasis. 
Material and Methods: The study included 150 females, within child-bearing periods, complaining of valvovaginal candidiasis. Candida strains were isolated, and identified by conventional microbiological methods; and confirmed by Viteck-2 System. The sensitivity of the isolates to voriconazole was performed, via the Disc diffusion method. Resistant strains were then subjected to minimum inhibitory concentrations (MIC) investigation of voriconazole alone, and in combination with a Kombucha tea culture via the broth micro-dilution method in concentrations ranging from 0.0048 to 10 μg/ml. The ability of voriconazole, with and without Kombucha, to eradicate Candida biofilms were investigated using a crystal violet absorbance assay. 
Results: Eighty-nine strains were isolated. From these 60 isolates showed variable resistance patterns (57 were voriconazole resistant, and 3 had dose-dependent susceptability). Kombucha significantly decreased the MIC50 of voriconazole against all strains from 5 to 0.625 μg/ml (p-value<0.01); additionally, MIC90 were reduced from 10 to 1.25 μg/ml (p-value =0.000). Voriconazole at a concentration of 0.156 μg/ml succeeded in eradicating biofilms formed by 18 strains after adding a Kombucha tea supernatant versus zero strains when using Voriconazole alone. 
Conclusion: Kombucha Black tea cultures could be promising antifungal agents in the treatment of vulvovaginal candidiasis.
 
5. Publisher Organizing agency, location Faculty of Medicine, Prince of Songkla University
 
6. Contributor Sponsor(s)
 
7. Date (YYYY-MM-DD) 2023-05-18
 
8. Type Status & genre Peer-reviewed Article
 
8. Type Type
 
9. Format File format PDF
 
10. Identifier Uniform Resource Identifier https://jhsmr.org/index.php/jhsmr/article/view/933
 
10. Identifier Digital Object Identifier (DOI) http://dx.doi.org/10.31584/jhsmr.2023933
 
11. Source Title; vol., no. (year) Journal of Health Science and Medical Research; Vol 41, No 4 (2023): Jul-Aug
 
12. Language English=en en
 
13. Relation Supp. Files
 
14. Coverage Geo-spatial location, chronological period, research sample (gender, age, etc.)
 
15. Rights Copyright and permissions Copyright (c) 2023 Journal of Health Science and Medical Research Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.