Anti-Gastric Ulcer Activity of the Water Extract from Payawanorn (Pseuderanthemum Palatiferum)
Abstract
Material and Methods: Evaluation of anti-gastric ulcer activity of PPE was performed using three induced gastric ulcers models: (1) ethanol/hydrochloric acid (EtOH/HCl), (2) restraint water immersion stress and (3) indomethacin. Ulcer indices were determined by microscopic examination (10X). Pylorus ligation and gastric-wall mucus determination were used to investigate the mechanism of anti-gastric ulcer activity of PPE.
Results: Oral administration of PPE (150, 300 and 600 mg/kg) significantly inhibited gastric ulcer formation induced by EtOH/HCl, by water immersion restraint stress, and by indomethacin in rats (p-value<0.05). PPE at 600 mg/kg showed a high percent inhibition of gastric ulcer formation in all models. In the pylorus-ligated model, pretreatment with PPE had no effect on pH, acidity output or the gastric acid secretion rate, demonstrating that PPE lacks an anti-secretory effect. Gastric wall mucus was markedly preserved by pretreatment PPE at 600 mg/kg (18.91±2.57 μg alcian blue/g wet stomach) after ethanol-induced ulcer when compared to that the control group (12.38±1.02 μg alcian blue/ g wet stomach).
Conclusion: PPE possesses an anti-gastric ulcer effect related to the preservation of gastric mucus, supporting the traditional use of P. palatiferum to treat gastric ulcers.
Keywords
Full Text:
PDFReferences
Padee P, Nualkaew S. Current information of medicinal plants: Pseuderanthemum palatiferum (Ness) Radlk. J Health Sci [Thai]. 2009;18(1):131-8.
Dieu HK, Loc CB, Yamasaki S, Hirata Y. The ethnobotanical and botanical study on Pseuderanthemum palatiferum as a new medicinal plant in the Mekong Delta of Vietnam. Jpn Agric Res Q. 2005;39(3):191-6. doi: 10.6090/jarq.39.191.
Mai HD, Minh HN, Pham VC, Bui KN, Nguyen VH, Chau VM. Lignans and other constituents from the roots of the Vietnamese medicinal plant Pseuderanthemum palatiferum. Planta Med. 2011;77(9):951-4. doi: 10.1055/s-0030-1250683.
Petsangkrit N, Kittipongpatana N. Establishment of Pseuderanthemum palatiferum (Ness) Radlk callus culture and screening of secondary metabolite production. Int J Pharm Pharm Sci. 2016;8(1):275-80.
Chayarop K, Peungvicha P, Wongkrajang Y, Chuakul W, Amnuoypol S, Temsiririrkkul R. Pharmacognostic and phytochemical investigations of Pseuderanthemum palatiferum (Nees) Radlk. ex Lindau Leaves. Pharmacogn J. 2011;3(23):18-23. doi: 10.1016/S0975-3575(11)80078-7.
Khonsung P, Panthong A, Chiranthanut N, Intahphuak S. Hypotensive effect of the water extract of the leaves of Pseuderanthemum palatiferum. J Nat Med. 2011;65(3-4):551-8. doi: 10.1007/s11418-011-0540-z.
Giang PM, Son PT. Phytochemical study on Pseuderanthemum (Nees) RADLK. Acanthaceae. J Chem [Vietnam]. 2003;41:115-8.
Mota KS, Dias GE, Pinto ME, Luiz-Ferreira A, Souza-Brito AR, Hiruma-Lima CA, et al. Flavonoids with gastroprotective activity. Molecules. 2009;14(3):979-1012. doi: 10.3390/molecules14030979.
Mizui T, Doteuchi M. Effect of polyamines on acidified ethanol-induced gastric lesions in rats. Jpn J Pharmacol. 1983;33(5):939-45. doi: 10.1254/jjp.33.939.
Sohn YA, Hwang SA, Lee SY, Hwang IY, Kim SW, Kim SY, et al. Protective effect of liriodendrin isolated from Kalopanax pictus against gastric injury. Biomol Ther (Seoul). 2015;23(1):53-9. doi: 10.4062/biomolther.2014.103.
Takagi K, Kasuya Y, Watanabe K. Studies on the drugs for peptic ulcer. A reliable method for producing stress ulcer in rats. Chem Pharm Bull (Tokyo). 1964;12(4):465-72. doi: 10.1248/cpb.12.465.
Kudryavtsev KV, Markevich AO, Virchenko OV, Falalyeyeva TM, Beregova TV, Ostapchenko LI, et al. Pharmacological correction of stress-induced gastric ulceration by novel small-molecule agents with antioxidant profile. Sci World J. 2014;2014:217039. doi: 10.1155/2014/217039.
Djahanguiri B. The production of acute gastric ulceration by indomethacin in the rat. Scand J Gastroenterol. 1969;4(3):265-7. PMID: 5346672
Nwafor PA, Okwuasaba FK, Binda LG. Antidiarrhoeal and antiulcerogenic effects of methanolic extract of Asparagus pubescens root in rats. J Ethnopharmacol. 2000;72(3):421-7. doi: 10.1016/s0378-8741(00)00261-0.
Shay H, Komarov SA, Fels SS, Meranze D, Gruenstein M, Siplet H. A simple method for the uniform production of gastric ulceration in the rat. Gastroenterology. 1945;5:43-61.
Souza R, Cardoso M, Menezes C, Silva J, De Sousa D, Batista J. Gastroprotective activity of alpha-terpineol in two experimental models of gastric ulcers in rats. DARU J Pharm Sci. 2011;19(4):277-81. PMID: 22615669
Corne SJ, Morrissey SM, Woods RJ. Proceedings: a method for the quantitative estimation of gastric barrier mucus. J Physiol. 1974;242(1):116P-117P. PMID: 4142046
Tarique M, Siddiqui HH, Khushtar M, Rahman MA. Protective effect of hydro-alcoholic extract of Ruta graveolens Linn. leaves on indomethacin and pylorus ligation-induced gastric ulcer in rats. J Ayurveda Integr Med. 2016;7(1):38-43. doi: 10.1016/j.jaim.2015.09.002.
Rujjanawate C, Kanjanapothi D, Amornlerdpison D, Pojanagaroon S. Anti-gastric ulcer effect of Kaempferia parviflora. J Ethnopharmacol. 2005;102(1):120-2. doi: 10.1016/j.jep.2005.03.035.
Rozza AL, Moraes Tde M, Kushima H, Tanimoto A, Marques MO, Bauab TM, et al. Gastroprotective mechanisms of Citrus lemon (Rutaceae) essential oil and its majority compounds limonene and beta-pinene: involvement of heat-shock protein-70, vasoactive intestinal peptide, glutathione, sulfhydryl compounds, nitric oxide and prostaglandin E(2). Chem Biol Interact. 2011;189(1-2):82-9. doi: 10.1016/j.cbi.2010.09.031.
Perez Y, Oyarzabal A, Mas R, Molina V, Jimenez S. Protective effect of D-002, a mixture of beeswax alcohols, against indomethacin-induced gastric ulcers and mechanism of action. J Nat Med. 2013;67(1):182-9. doi: 10.1007/s11418-012-0670-y.
Soni H PS, Paul A, Patel G. Evaluation of gastro-protective effect of pep-up tablet and pep-up syrup on water immersion plus restraint stress-induced gastric ulcer in rats. Int J Herb Med. 2014;2(2):27-30.
Coelho-de-Souza AN, Lahlou S, Barreto JE, Yum ME, Oliveira AC, Oliveira HD, et al. Essential oil of Croton zehntneri and its major constituent anethole display gastroprotective effect by increasing the surface mucous layer. Fundam Clin Pharmacol. 2013;27(3):288-98. doi: 10.1111/j.1472-8206.2011.01021.x.
Refbacks
- There are currently no refbacks.
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.