Curcumin Enhances the Reduction of Keloid Fibroblast Viability Induced by Blue Light
Abstract
Objective: This study aims to determine the effects of blue light or curcumin alone and its combination on keloid fibroblast viability.
Material and Methods: Human keloid fibroblasts were divided into different groups: untreated controls, blue light irradiation at 30 J/cm², curcumin treatment (1.25, 2.5, 5, 10, and 20 µg/mL), and curcumin combined with blue light.
Results: Cell viability was assessed using an 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, and curcumin’s half-maximal inhibitory concentration (IC50) was calculated through regression analysis. Blue light at 30 J/cm2 significantly reduced keloid fibroblast viability to 61.99%, while curcumin at 20 µg/mL decreased viability to 72.01%. When combined with blue light, curcumin exhibited a dose-dependent effect: 5 µg/mL of curcumin reduced viability to 36.51%. In contrast, 10 and 20 µg/mL decreased viability to 15.94% and 15.85%, respectively—significantly greater reductions than curcumin alone. Blue light enhanced curcumin’s effects, lowering its IC50 from 30.95 µg/mL to 3.86 µg/mL.
Conclusion: The combination of blue light and curcumin demonstrated synergistic effects, achieving greater reductions in keloid fibroblast viability compared to either treatment alone.
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Smith N, Cha KB, Bichakjian C. Perioperative consideration in dermatologic surgery. In: Kang S, et al., editors. Fitzpatrick’s Dermatology. 9th ed. New York: McGraw-Hill; 2019;p.3273.
Choirunanda AF, Praharsini I. Profil gangguan kualitas hidup akibat keloid pada mahasiswa fakultas kedokteran udayana angkatan 2012-2014. J Med Udayana 2019;8:1–5.
Klomparens K, Simman R. Treatment of Keloids: A Meta-analysis of Intralesional Triamcinolone, Verapamil, and Their Combination. Plast Reconstr Surg Glob Open 2022;10:e4075.
Sadowska M, Narbutt J, Lesiak A. Blue light in dermatology. Life 2021;11:670.
Krassovka JM, Suschek CV, Prost M, Grotheer V, Schiefer JL, Demir E, et al. The impact of non-toxic blue light (453 nm) on cellular antioxidative capacity, TGF-β1 signaling, and myofibrogenesis of human skin fibroblasts. J Photochem Photobiol B 2020;209:111952.
Li JY, Zhang K, Xu D, Zhou WT, Fang WQ, Wan YY, et al. Mitochondrial fission is required for blue light-induced apoptosis and mitophagy in retinal neuronal R28 cells. Front Mol Neurosci 2018;11:432.
Ge G, Wang Y, Xu Y, Pu W, Tan Y, Liu P, et al. Induced skin aging by blue-light irradiation in human skin fibroblasts via TGF-β, JNK and EGFR pathways. J Dermatol Sci 2023;111:52–9.
Alkahtani S, S Al-Johani N, Alarifi S, Afzal M, Cytotoxicity mechanisms of blue-light-activated curcumin in T98G cell line: inducing apoptosis through ROS-dependent downregulation of MMP pathways. Int J Mol Sci 2023;24:3842.
Strazzi Sahyon HB, Silva PP da, Oliveira MS de, Cintra LTA, Dezan-Júnior E, Gomes-Filho JE, et al. Influence of curcumin photosensitizer in photodynamic therapy on the mechanical properties and push-out bond strength of glass-fiber posts to intraradicular dentin. Photodiagnosis Photodyn Ther 2019;25:376–81.
Vollono L, Falconi M, Gaziano R, Iacovelli F, Dika E, Terracciano C, et al. Potential of curcumin in skin disorders. Nutrients 2019;11:2169.
Park J Il, Kim SJ, Kim YJ, Lee SJ. Protective role of Caesalpinia sappan extract and its main component brazilin against blue light–induced damage in human fibroblasts. J Cosmet Dermatol 2022;21:7025–34.
Jalilian E, Abolhasani-Zadeh F, Afgar A, Samoudi A, Zeinalynezhad H, Langroudi L. Neutralizing tumor-related inflammation and reprogramming of cancer-associated fibroblasts by curcumin in breast cancer therapy. Sci Rep 2023;13:20770.
Do XH, Hoang MHT, Vu AT, Nguyen LT, Bui DTT, Dinh DT, et al. Differential cytotoxicity of Curcumin-loaded micelles on human tumor and Stromal cells. Int J Mol Sci 2022;23:12362.
Montero P, Roger I, Milara J, Cortijo J. Damaging effects of UVA, blue light, and infrared radiation: in vitro assessment on a reconstructed full-thickness human skin. Front Med (Lausanne) 2023;10.
Brüning AKE, Schiefer JL, Fuchs PC, Petzsch P, Köhrer K, Suschek CV, et al. Low-dose blue light (420 nm) reduces Metabolic activity and inhibits proliferation of human dermal fibroblasts. Life 2023;13:331.
Polat E, Kang K. Natural photosensitizers in antimicrobial photodynamic therapy. Biomedicines 2021;9:584.
Vázquez Alberdi L, Martínez-Busi M, Arrarte E, Echeverry C, Calero M, Kun A. A low dose of curcumin-PDA nanoparticles improves viability and proliferation in endoneurial fibroblasts and Schwann cell cultures. Discover Nano 2024;19:81.
Lee JH, Kim DH, Kim M, Jung KH, Lee KH. Mitochondrial ROS-mediated Metabolic and Cytotoxic effects of Isoproterenol on Cardiomyocytes are p53-dependent and reversed by Curcumin. Molecules 2022;27:1346.
Scharstuhl A, Mutsaers HAM, Pennings SWC, Szarek WA, Russel FGM, Wagener FADTG. Curcumin-induced fibroblast apoptosis and in vitro wound contraction are regulated by antioxidants and heme oxygenase: implications for scar formation. J Cell Mol Med 2009;13:712–25.
Ma J, Ma S yu, Ding C hua. Curcumin reduces cardiac fibrosis by inhibiting myofibroblast differentiation and decreasing transforming growth factor β1 and matrix metalloproteinase 9/tissue inhibitor of metalloproteinase 1. Chin J Integr Med 2017;23:362–9.
Strazzi-Sahyon HB, Cintra LTA, Nakao JM, Takamiya AS, Queiroz IOA, Dos Santos PH, et al. Cytotoxicity of root canal irrigating solutions and photodynamic therapy using curcumin photosensitizer. Photodiagnosis Photodyn Ther 2022;38:102795.
Zheng N, Zhou M, He Y, Xu H, Chen X, Duan Z, et al. Low curcumin concentrations combined with blue light inhibits cutibacterium acnes biofilm-induced inflammatory response through suppressing MAPK and NF-B in keratinocytes. Photodiagnosis Photodyn Ther 2022;40:103204.
Manoil D, Filieri A, Gameiro C, Lange N, Schrenzel J, Wataha JC, et al. Flow cytometric assessment of Streptococcus mutans viability after exposure to blue light-activated curcumin. Photodiagnosis Photodyn Ther 2014;11:372–9.
Woźniak M, Nowak M, Lazebna A, Więcek K, Jabłońska I, Szpadel K, et al. The comparison of in vitro Photosensitizing efficacy of Curcumin-loaded Liposomes following photodynamic therapy on Melanoma MUG-Mel2, Squamous cell Carcinoma SCC-25, and normal Keratinocyte HaCaT cells. Pharmaceuticals 2021;14:374.
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