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Home > Vol 37, No 2 (2019) > Suksai

Effects of Trehalose and Sucrose on Human Sperm Motility, Vitality and Morphology after Cryopreservation

Manaphat Suksai, Kriengsak Dhanaworavibul

Abstract

Objective: To compare human sperm motility, vitality and morphology after cryopreservation among sucrose, and different trehalose concentrations.
Material and Methods: A total number of 124 normozoospermic semen samples were collected. Each semen sample was divided into 4 portions, and cryopreserved in a human sperm-preserving medium along with cryoprotectants, including; 50 milimolar (mM) trehalose, 100 mM trehalose, 200 mM trehalose and 50 mM sucrose, respectively. All semen samples were frozen by using a vapor phase method. Post-thawed sperm motility, vitality and morphology were assessed. R program was used for data analysis. A p-value of <0.05 was considered statistically significant.
Results: Post-thawed semen evaluation indicated that 50 mM trehalose was better than 50 mM sucrose in all sperm parameters, which included progressive motility (p-value=0.037, total motility (p-value<0.001), vitality (p-value<0.001) and morphology (p-value<0.001). The sperm parameters were not significantly different among 100 mM trehalose, 200 mM trehalose and 50 mM sucrose.
Conclusion: The use of 50 mM trehalose, as non-permeating cryoprotectant, showed superior post-thaw sperm parameters over sucrose, and other trehalose concentrations.

 Keywords

cryopreservation; human sperm; sucrose; trehalose

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DOI: http://dx.doi.org/10.31584/jhsmr.201945

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About The Authors

Manaphat Suksai
Department of Obstetrics and Gynecology, Faculty of Medicine, Prince of Songkla University, Songkhla 90110,
Thailand

Kriengsak Dhanaworavibul
Department of Obstetrics and Gynecology, Faculty of Medicine, Prince of Songkla University, Songkhla 90110,
Thailand

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