Characteristics of Buccal Fat Adipose-Derived Stem Cells Cultured in Autologous Human Serum- and Fetal Bovine Serum-Supplemented Media: A Comparative Study
Abstract
Objective: The characteristics of adipose-derived stem cells (ADSC), isolated from buccal fat pads when cultured in media supplemented, with either autologous human serum (AHS group) or fetal bovine serum (FBS group) were compared.
Material and Methods: Buccal fat tissue was harvested from six patients, who had undergone surgical removal of their maxillary third molars or underwent orthognathic surgeries. ADSC were isolated from the tissue, via an enzymatic digestion method and cultured in the media of the AHS and FBS groups (n=6/group). Colony forming units-fibroblast (CFU-F), immune-phenotyping markers, growth and multi-differentiation of the cells from both groups were compared.
Results:The number of CFU-F and the cell growth of the AHS group were significantly greater than those of the FBS group (p-value<0.05). The expressions of the mesenchymal and hematopoietic stem cell markers of both groups were not statistically different. The cells of both groups had the potential for adipogenesis, chondrogenesis and osteogenesis when cultured in inductive conditions.
Conclusion: The immunophenotype and multi-differentiation of ADSC, which were cultured in AHS- and FBS- media, were not different. However, the AHS medium could support the capacity for forming colonies and growth of the cells better than the FBS medium.
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Kern S, Eichler H, Stoeve J, Kluter H, Bieback K. Comparative analysis of mesenchymal stem cells from bone marrow, umbilical cord blood, or adipose tissue. Stem Cells 2006;24:1294-301.
Farre-Guasch E, Marti-Page C, Hernadez-Alfaro F, Klein-Nulend J, Casals N. Buccal fat pad, an oral access source of human adipose stem cells with potential for osteochondral tissue engineering: an in vitro study. Tissue Eng Part C Methods 2010;16:1083-94.
Kishimoto N, Momota Y, Hashimoto Y, Tatsumi S, Ando K, Omasa T, et al. The osteoblastic differentiation ability of human dedifferentiated fat cells is higher than that of adipose stem cells from the buccal fat pad. Clin Oral Investig 2014;18:1893-901.
Suzuki E, Fujita D, Takahashi M, Oba S, Nishimatsu H. Adipose tissue-derived stem cells as a therapeutic tool for cardiovascular disease. World J Cardiol 2015;7:454-65.
Karantalis V, Hare JM. Use of mesenchymal stem cells for therapy of cardiac disease. Circ Res 2015;116:1413-30.
Strem BM, Hicok KC, Zhu M, Wulur I, Alfonso Z, Schreiber RE, et al. Multipotential differentiation of adipose tissue-derived stem cells. Keio J Med 2005;54:132-41.
Broccaioli E, Niada S, Rasperini G, Ferreira LM, Arrigoni E, Yenagi V, et al. Mesenchymal Stem Cells from Bichat’s Fat Pad: In Vitro Comparison with Adipose-Derived Stem Cells from Subcutaneous Tissue Biores Open Access 2013;2:107-17.
Niada S, Ferreira LM, Arrigoni E, Addis A, Campagnol M, Broccaioli E, Brini AT. Porcine adipose-derived stem cells from buccal fat pad and subcutaneous adipose tissue for future preclinical studies in oral surgery. Stem Cell Res Ther 2013;4:148.
Arpornmaeklong P, Srinual N, Pripatnanont P. The Effect of Cell Source on Mesenchymal Stem Cell Behavior and Osteogenic Differentiation of Buccal Fat Pad and Dental Pulp Stem Cells, an In Vitro Study. J Health Sci Med Res 2022;40:671-84.
Tekkatte C, Gunasingh GP, Cherian KM, Sankaranarayanan K. “Humanized” stem cell culture techniques: the animal serum controversy. Stem Cells Int 2011;2011:504723.
Oeller M, Laner-Plamberger S, Krisch L, Rohde E, Strunk D, Schallmoser K. Human Platelet Lysate for Good Manufacturing Practice-Compliant Cell Production. Int J Mol Sci 2021;22:5178.
Palombella S, Perucca Orfei C, Castellini G, Gianola S, Lopa S, Mastrogiacomo M, et al. Systematic review and meta-analysis on the use of human platelet lysate for mesenchymal stem cell cultures: comparison with fetal bovine serum and considerations on the production protocol. Stem Cell Res Ther 2022;13:142.
Chase LG, Lakshmipathy U, Solchaga LA, Rao MS, Vemuri MC. A novel serum-free medium for the expansion of human mesenchymal stem cells. Stem Cell Res Ther 2010;1:8.
Al-Saqi SH, Saliem M, Asikainen S, Quezada HC, Ekblad Å, Hovatta O, et al. Defined serum-free media for in vitro expansion of adipose-derived mesenchymal stem cells. Cytotherapy 2014; 16:915–26.
Gottipamula S, Ashwin KM, Muttigi MS, Kannan S, Kolkundkar U, Seetharam RN. Isolation, expansion and characterization of bone marrow-derived mesenchymal stromal cells in serum-free conditions. Cell Tissue Res 2014;356:123–35.
Kandoi S, Patra B, Vidyasekar P, Sivanesan D, Verma RS. Evaluation of platelet lysate as a substitute for FBS in explant and enzymatic isolation methods of human umbilical cord MSCs. Sci Rep 2018;8:12439.
Swamynathan P, Venugopal P, Kannan S, Thej C, Kolkundar U, Bhagwat S, et al. Are serum-free and xeno-free culture conditions ideal for large scale clinical grade expansion of Wharton’s jelly derived mesenchymal stem cells? A comparative study. Stem Cell Res Ther 2014;5:88.
Burnouf T, Strunk D, Koh MBC, Schallmoser K. Human platelet lysate: replacing fetal bovine serum as a gold standard for human cell propagation? Biomaterials 2016;76:371–87.
Palombella S, Guiotto M, Higgins GC, Applegate LL, Rafoul W, Cherubino M, et al. Human platelet lysate as a potential clinical-translatable supplement to support the neurotrophic properties of human adipose-derived stem cells. Stem Cell Res Ther 2020;11:432.
Escobar CH, Chaparro O. Xeno-free extraction, culture, and cryopreservation of human adipose-derived mesenchymal stem cells: Xeno-free hASCs. Stem Cells Transl Med 2016;5:358–65.
Fani N, Ziadlou R, Shahhoseini M, Baghaban Eslaminejad M. Comparative epigenetic influence of autologous versus fetal bovine serum on mesenchymal stem cells through in vitro osteogenic and adipogenic differentiation. Exp Cell Res 2016;344:176-82.
Shahdadfar A, Fronsdal K, Haug T, Reinholt FP, Brinchmann JE. In vitro expansion of human mesenchymal stem cells: choice of serum is a determinant of cell proliferation, differentiation, gene expression, and transcriptome stability. Stem Cells 2005;23:1357-66.
Yamamoto N, Isobe M, Negishi A, Yoshimasu H, Shimokawa H, Ohya K, et al. Effects of autologous serum on osteoblastic differentiation in human bone marrow cells. J Med Dent Sci 2003;50:63-9.
Matsuo A, Yamazaki Y, Takase C, Aoyagi K, Uchinuma E. Osteogenic potential of cryopreserved human bone marrow-derived mesenchymal stem cells cultured with autologous serum. J Craniofac Surg 2008;19:693-700.
Takeda A, Yamazaki Y, Baba K, Ishiguro M, Aoyagi K, Ikemoto S, et al. Osteogenic potential of human bone marrow-derived mesenchymal stromal cells cultured in autologous serum: a preliminary study. J Oral Maxillofac Surg 2012;70:e469-76.
Arpornmaeklong P, Sutthitrairong C, Jantaramanant P, Pripatnanont P. Allogenic human serum, a clinical grade serum supplement for promoting human periodontal ligament stem cell expansion. J Tissue Eng Regen Med 2018;12:142-52.
Dominici M, Le Blanc K, Mueller I, Slaper-Cortenbach I, Marini F, Krause D, et al. Minimal criteria for defining multipotent mesenchymal stromal cells. The International Society for Cellular Therapy position statement. Cytotherapy 2006;8:315-7.
Asatrian G, Pham D, Hardy WR, James AW, Peault B. Stem cell technology for bone regeneration: current status and potential applications. Stem Cells Cloning 2015;8:39-48.
Lv FJ, Tuan RS, Cheung KM, Leung VY. Concise review: the surface markers and identity of human mesenchymal stem cells. Stem Cells 2014;32:1408-19.
Billon N, Iannarelli P, Monteiro MC, Glavieux-Pardanaud C, Richardson WD, Kessaris N, et al. The generation of adipocytes by the neural crest. Development 2007; 134: 2283-92.
Cuevas-Diaz Duran R, Gonzalez-Garza MT, Cardenas-Lopez A, Chavez-Castilla L, Cruz-Vega DE, Moreno-Cuevas JE. Age-related yield of adipose-derived stem cells bearing the low-affinity nerve growth factor receptor. Stem Cells Int 2013;2013:372164.
Quirici N, Scavullo C, de Girolamo L, Lopa S, Arrigoni E, Deliliers GL, et al. Anti-L-NGFR and -CD34 monoclonal antibodies identify multipotent mesenchymal stem cells in human adipose tissue. Stem Cells Dev 2010;19:915-25.
Slukvin, II, Vodyanik M. Endothelial origin of mesenchymal stem cells. Cell Cycle 2011;10:1370-3.
Kawashima N. Characterisation of dental pulp stem cells: a new horizon for tissue regeneration? Arch Oral Biol 2012;57:1439-58.
Oberbauer E, Steffenhagen C, Wurzer C, Gabriel C, Redl H, Wolbank S. Enzymatic and non-enzymatic isolation systems for adipose tissue-derived cells: current state of the art. Cell Regen 2015;4:7.
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