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Home > Vol 43, No 2 (2025) > Ahmed

Optimizing an Infusion Method of Producing Cosmos Caudatus Encapsulated In Exosomes as A Nanocarrier for Drug Delivery

Alhakam Ahmed, Wan Amir, Wan Nazatul Shima Shahidan

Abstract

Objective: This study aimed to propose different encapsulation methods of a medicinal plant extract Cosmos Caudatus (CC), into HSC-2-derived exosomes.
Material and Methods: Encapsulation of HSC-2-derived exosomes was achieved via internal and external infusion. The internal infusion was achieved by treating HSC-2 cells with CC extract and isolating the exosomes from its media (M1_ExoCC). The external infusion was performed by dissolving CC extract with HSC-2 purified plain culture media (M2_ExoCC), direct incubation of isolated exosomes suspended in PBS with CC extract (M3_ExoCC), freeze-thaw incubation of purified HSC-2 media containing CC extract (M4_ExoCC), and freeze-thaw incubation of PBS suspended exosome with CC extract (M5_ExoCC).
Results: M1_ExoCC and M2_ExoCC were the most advantageous encapsulation methods in terms of yield, stability and solubility.
Conclusion: It was determined that the M1_ExoCC and M2_ExoCC methods yielded the most favorable outcomes across all parameters. The cost-effectiveness and simplicity of these methods make them particularly appealing for scaling up production and translating research findings into clinical practice.

 Keywords

Cosmos Caudatus; drug delivery; exosomes; nanocarrier; solubility; stability

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DOI: http://dx.doi.org/10.31584/jhsmr.20241123

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2020
Acceptance rate: 52%
2021
Acceptance rate: 27.8%
2022 (March)
Acceptance rate: 15.6%
2023 (June)
Acceptance rate: 23.6%
2024 (June)
Acceptance rate: 19%


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About The Authors

Alhakam Ahmed
School of Dental Sciences, Universiti Sains Malaysia, 16150 Kubang Kerian, Kelantan,
Malaysia

Wan Amir
School of Health Sciences, Universiti Sains Malaysia, 16150 Kubang Kerian, Kelantan,
Malaysia

Wan Nazatul Shima Shahidan
School of Dental Sciences, Universiti Sains Malaysia, 16150 Kubang Kerian, Kelantan,
Malaysia

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